LOCUS       40924_31695       13034 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pMpGWB312 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T.
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha
  JOURNAL   PLoS ONE 10 (9), E0138876 (2015)
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13034)
  AUTHORS   Nishihama R, Ishizaki K, Kohchi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, 
            Graduate School of Biostudies; Kitashirakawa-oiwake-cho, Sakyo-ku, 
            Kyoto, Kyoto 606-8502, Japan URL :http://www.lif.kyoto-u.ac.jp/e/
REFERENCE   3  (bases 1 to 13034)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2015"; volume: "10"; issue: "9"; pages: "E0138876"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-JUN-2015) Contact:Ryuichi Nishihama Kyoto University, Graduate 
            School of Biostudies"; volume: " Kitashirakawa-oiwake-cho, Sakyo-ku,
            Kyoto, Kyoto 606-8502, Japan URL :http"; pages: 
            "//www.lif.kyoto-u.ac.jp/e"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        484..514
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             568..1224
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1569..1871
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1915..2039)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2050..2925
                     /codon_start=1
                     /product="ligand binding domain of the glucocorticoid
                     receptor"
                     /label=ligand binding domain of the glucocorticoid rec
                     /note="region_name: GR; glucocorticoid receptor, a member
                     of the nuclear receptor superfamily"
                     /protein_id="BAS49915.1"
                     /translation="MASEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPT
                     LVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAIPGFRNLHLD
                     DQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFV
                     SSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNS
                     SQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNG
                     NIKKLLFHQK"
     terminator      2941..3193
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3227..3243)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3251..3267
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3275..3305)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3320..3341)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    complement(3426..3601)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3609..5645)
                     /codon_start=1
                     /gene="mALS"
                     /product="acetolactate synthase"
                     /label=mALS
                     /note="from Marchantia polymorpha; contains triple
                     amino-acid subustitutions (P207S/R208S/W582L)"
                     /protein_id="BAS49916.1"
                     /translation="MASISGCAAVPLSIASLEKVQPSARSCCKGAKELKVRSGFFSGAA
                     LRGHAYNTSRKTERSLEVKAQAVSEKIQSAVDVSGPRLSSGKSLVAPTPSDFQSRYKDD
                     EPRKGADILVEALEREGIRTVFAYPGGASMEIHQALTRSSSIRNILCRHEQGEIFAAEG
                     YAKSSGRVGVCIATSGPGATNLVTGLADALLDSVPLVAITGQVSSRMIGTDAFQETPIV
                     EVTRSITKHNYLVMNIDDIPRVIREAFFLAASGRPGPVLVDIPKDVQQQMAIPDWSQPM
                     QLKGYLQRLPRPPEPTLIEQIIRLISSSKKPVVYSGGGCNHASEELREFVELTGIPITS
                     TLMGLGAYPESDPLSLKMLGMHGTVYANYAVDQADLLLAFGVRFDDRVTGKLETFASRS
                     SIVHIDIDPAEIGKNKQPHVSMCADVKPALSEINALLKSQKRTFDFSNWVAELEEQKQK
                     WPMKFPVFEDAIAPQSAIQTLYELTGGNAIISTGVGQHQMWAAQWFKYNEPRRWLTSGG
                     LGAMGFGLPSALGAAATQPDIPVIDIDGDGSFVMNVQELATIFVEKLPVKIMILNNQHL
                     GMVVQLEDRFYKANRAHTYLGDPDHEEQIYPDFCTVADGFKVPNARVVKKDELRAAIQT
                     MLDSPGPYLLDVIVPHQEHVLPMIPGGASFKEIITEGDGRTIY"
     gene            complement(3609..5645)
                     /gene="mALS"
                     /label=mALS
     promoter        complement(5685..6361)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     misc_feature    complement(6556..6580)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7101..7889
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8138..8726
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8912..9052)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9396..9590)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9659..10729)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11161..11787)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacaggga
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 gataacagca tggctagtga agctcgaaaa acaaagaaaa aaatcaaagg gattcagcaa
     2101 gccactgcag gagtctcaca agacacttcg gaaaatccta acaaaacaat agttcctgca
     2161 gcattaccac agctcacccc taccttggtg tcactgctgg aggtgattga acccgaggtg
     2221 ttgtatgcag gatatgatag ctctgttcca gattcagcat ggagaattat gaccacactc
     2281 aacatgttag gtgggcgtca agtgattgca gcagtgaaat gggcaaaggc gataccaggc
     2341 ttcagaaact tacacctgga tgaccaaatg accctgctac agtactcatg gatgtttctc
     2401 atggcatttg ccctgggttg gagatcatac agacaatcaa gtggaaacct gctctgcttt
     2461 gctcctgatc tgattattaa tgagcagaga atgtctctac cctgcatgta tgaccaatgt
     2521 aaacacatgc tgtttgtctc ctctgaatta caaagattgc aggtatccta tgaagagtat
     2581 ctctgtatga aaaccttact gcttctctcc tcagttccta aggaaggtct gaagagccaa
     2641 gagttatttg atgagattcg aatgacttat atcaaagagc taggaaaagc catcgtcaaa
     2701 agggaaggga actccagtca gaactggcaa cggttttacc aactgacaaa acttctggac
     2761 tccatgcatg aggtggttga gaatctcctt acctactgct tccagacatt tttggataag
     2821 accatgagta ttgaattccc agagatgtta gctgaaatca tcactaatca gataccaaaa
     2881 tattcaaatg gaaatatcaa gaagctgctg tttcatcaaa aatagagctc gaatttcccc
     2941 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     3001 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     3061 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     3121 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     3181 atgttactag atcgggaatt ggttccggaa ccaattcgta atcatggtca tagctgtttc
     3241 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     3301 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aggctgaatt aggcgcgcct
     3361 atttctgaat tcaacacatt gcggacgttt ttaatgtact gaattaacgc cgaattaatt
     3421 cgggggatct ggattttagt actggatttt ggttttagga attagaaatt ttattgatag
     3481 aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga
     3541 aaccctatag gaaccctaaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     3601 actcgaagtt agtatatagt ccttccatca ccctctgtga taatctcctt aaaacttgct
     3661 cccccaggaa tcataggcag aacgtgctcc tggtggggaa ctatcacatc cagtaaataa
     3721 ggaccgggag aatctagcat agtttgaatt gctgctctta gttcatcctt cttcaccaca
     3781 cgcgcatttg gcaccttgaa accgtctgca actgtacaga agtcggggta gatctgctcc
     3841 tcgtgatctg gatcacctaa gtaagtatgt gctctgttgg ccttgtagaa tctatcctcc
     3901 aattgtacga ccattcctag atgttggttg tttaagatca tgatcttcac gggtaacttc
     3961 tccacaaaaa ttgtagccaa ctcctgtacg ttcatcacaa agctaccatc gccatcgatg
     4021 tcgatcacag gaatatctgg ttgagttgcg gcagccccca aagcagaagg aagtccgaat
     4081 cccatggctc caagacctcc agatgtcaac caacgccgcg gttcgttgta cttgaaccac
     4141 tgggctgccc acatttgatg ttggccaaca ccagtagata tgatggcatt tccaccagtg
     4201 agttcgtaca gcgtctgtat ggcagattga ggagcaatgg catcctcgaa tacgggaaac
     4261 ttcattggcc atttctgctt ttgttcttct agttctgcca cccagttgga gaagtcgaaa
     4321 gtcctctttt gactcttcaa gagggcattg atttcactta gcgcaggttt cacatcagca
     4381 cacatggaca catggggctg tttgttcttt ccaatttcag cagggtcaat atcgatatga
     4441 acgatgctgg acctgctagc aaaagtctcc agtttaccag tcactcgatc gtcgaaacgc
     4501 actccaaatg ccaacaacag atctgcttgg tccacggcgt agttggcgta aacagttcca
     4561 tgcattccaa gcattttcaa acttaatgga tcagactcgg gatacgctcc caaacccatt
     4621 aaagtcgatg tgataggaat tccggtaagc tcgacaaatt ccctcaactc ttcgctggcg
     4681 tgattgcaac ctcctccaga atacactaca ggcttcttag aactagaaat gagtcttata
     4741 atctgctcga ttaacgttgg ttccggtggc cgaggcagtc gttgtaagta gcccttcagt
     4801 tgcattggct gagaccaatc tggaatagcc atttgctgtt gcacgtcctt gggaatatct
     4861 accaagactg ggcccggcct acctgaagct gccaaaaaga aagcctccct gattacgcga
     4921 gggatgtcat caatgttcat aacaaggtaa ttgtgcttgg tgatcgatct cgtaacctcc
     4981 acaattggag tttcttgaaa agcatcggta ccaatcatcc tactggatac ttgacctgta
     5041 atggccacta gtggcacact gtccaagagg gcatcagcga gcccggtaac aagattggtc
     5101 gctccaggac cggatgtggc tatacaaacc cctactcgtc cagacgattt agcataacct
     5161 tcagcagcga aaatctcccc ctgctcgtgg cgacagagaa tgttgcggat gctgctggaa
     5221 cgtgtaagag cctggtgaat ctccatagac gctccgccgg gataagcgaa caccgttcta
     5281 atcccttctc gctcaagagc ctccaccaaa atgtcggctc ctttgcgggg ctcgtcatcc
     5341 ttgtagcgag actgaaagtc ggacggggtt ggtgcaacga gtgacttccc gctgctaagc
     5401 cgtggtccac tcacatcgac tgcagactgg atcttctcgc taacagcttg ggctttcact
     5461 tccagtgaac gctcagtctt ccgagaggtg ttgtatgcat gtcccctcag agcggctccc
     5521 gagaagaaac cactcctcac ctttagctct ttagctccct tgcaacaaga ccttgccgat
     5581 ggctgcacct tctccaggct cgctatgctc aaaggcacag ctgcgcatcc actaattgac
     5641 gccatgctcg agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc
     5701 aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg
     5761 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     5821 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     5881 gcagaggcat cttgaacgat agcctttcct ttatcgcaat tatggcattt gtaggtgcca
     5941 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     6001 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     6061 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
     6121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
     6181 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
     6241 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
     6301 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
     6361 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
     6421 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgaattc agaaataaat
     6481 tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg
     6541 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc
     6601 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg
     6661 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg
     6721 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt
     6781 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga
     6841 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt
     6901 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt
     6961 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     7021 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc
     7081 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat
     7141 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     7201 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     7261 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     7321 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     7381 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     7441 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     7501 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     7561 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     7621 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     7681 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     7741 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     7801 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga
     7861 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga
     7921 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac
     7981 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac
     8041 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact
     8101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8281 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8881 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8941 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     9001 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     9061 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     9121 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     9181 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt
     9241 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg
     9301 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt
     9361 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa
     9421 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat
     9481 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt
     9541 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac
     9601 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg
     9661 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag
     9721 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc
     9781 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct
     9841 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac
     9901 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct
     9961 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc
    10021 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg
    10081 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta
    10141 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga
    10201 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt
    10261 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat
    10321 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct
    10381 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt
    10441 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg
    10501 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc
    10561 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg
    10621 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac
    10681 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc
    10741 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg
    10801 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg
    10861 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca
    10921 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc
    10981 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct
    11041 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg
    11101 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca
    11161 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc
    11221 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg
    11281 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc
    11341 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc
    11401 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta
    11461 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa
    11521 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac
    11581 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac
    11641 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc
    11701 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt
    11761 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat
    11821 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt
    11881 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg
    11941 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc
    12001 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt
    12061 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg
    12121 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct
    12181 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc
    12241 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt
    12301 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg
    12361 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc
    12421 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg
    12481 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc
    12541 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt
    12601 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa
    12661 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt
    12721 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat
    12781 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac
    12841 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt
    12901 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt
    12961 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac
    13021 gaacggataa acct
//
